PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes

作者：Yu, Caihua; Xi, Lingling; Chen, Jin; Jiang, Qin; Yi, Hongbo; Wang, Yizhen; Wang, Xinxia^*

来源：Lipids, 2017, 52(11): 929-938.

DOI：10.1007/s11745-017-4302-x

• Porcine adipocytes
• Fatty acids
• Lipid accumulation
• Adipogenesis
• PPAR gamma phosphorylation

Dietary fatty acids have different effects on fat deposition in pigs. To clarify the underlying mechanisms of this difference, we compared the metabolism of palmitic (PAM, saturated), oleic (OLA, monounsaturated) and linoleic acid (LNA, polyunsaturated) in porcine adipocytes treated with 100 mu M PAM, OLA or LNA. We observed that the adipocytes incubated with LNA accumulated more lipids compared with those treated with PAM and OLA. We then probed the metabolism of these fatty acids in porcine adipocytes by using isotope-labelled fatty acids. The results showed that 42% of the [1-C-14] LNA, 34% of the [1-C-14] PAM and 28% of the [1-C-14] OLA were recovered in the cellular lipids. The gene expression analyses showed that LNA significantly increased the expression of adipogenesis- and oxidation-related genes including PPAR gamma, C/EBP alpha, ap2 and NRF1. In addition, the cells incubated with LNA showed a decreased Ser(112) phosphorylation in PPAR gamma compared to those incubated with PAM and OLA. Furthermore, when PPAR gamma Ser(112) phosphorylation was inhibited, no significant difference in the triacylglycerol contents in the adipocytes was observed. These results showed the dietary fatty acids had different metabolism pathways in porcine adipocytes, and LNA significantly promoted lipid accumulation, probably by regulating PPAR gamma phosphorylation in adipocytes.

• 出版日期2017-11
• 单位浙江大学