科研之友
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摘要
Objective: Advantages of introducing a salivary phosphoprotein homologue under standardized in vitro conditions to simulate the mineral-stabilizing properties of saliva have been proposed. This study longitudinally investigates the effects of casein, incorporated as a potential salivary phosphoprotein homologue in artificial saliva (AS) solutions with/without fluoride (F) on in vitro dentine lesion remineralization. Design: Thin sections of bovine root dentine were demineralized and allocated randomly into 6 groups (n = 18) having equivalent mineral loss (Delta Z) after transverse microradiography (TMR). The specimens were remineralized using AS solutions containing casein 0 mu g/ml, F 0 ppm (C-0-F-0); casein 0 mu g/ml, F 1 ppm (C-0-F-1); casein 10 mu g/ml, F 0 ppm (C-10-F-1); casein 10 mu g/ml, F 1 ppm (C-10-F-1); casein 100 mu g/ml, F 0 ppm (C-100-F-0) or casein 100 mu g/ml, F 1 ppm (C-100-F-1) for 28 days with TMR taken every 7 days. Results: Surface mineral precipitation, evident in group C-0-F-1 was apparently inhibited in groups with casein incorporation. Repeated measures ANOVA with Bonferroni correction revealed higher Delta Z for non F and non-casein groups than for their counterparts (p < 0.001). Subsequent multiple comparisons showed that mineral gain was higher (p < 0.001) with 10 mu g/ml casein than with 100 mu g/ml when F was present in the earlier stages of remineralization, with both groups achieving almost complete remineralization after 28 days. Conclusion: Casein is a potential salivary phosphoprotein homologue that could be employed for in vitro dentine remineralization studies. Concentration related effects may be clinically significant and thus must be further examined.
- 出版日期2016-8
