Determination of Plant Thiols by Liquid Chromatography Coupled with Coulometric and Amperometric Detection in Lettuce Treated by Lead(II) Ions

作者：Diopan Vaclav; Shestivska Violetta; Zitka Ondrej; Galiova Michaela; Adam Vojtech; Kaiser Jozef; Horna Ales; Novotny Karel; Liska Miroslav; Havel Ladislav; Zehnalek Josef; Kizek Rene^*

来源：Electroanalysis, 2010, 22(11): 1248-1259.

DOI：10.1002/elan.200900374

• Thiols
• Liquid chromatography
• Coulometric detection
• Amperometric detection
• Heavy metal
• Laser induced breakdown spectroscopy

The main aim of this paper is to utilize high performance liquid chromatography with electrochemical detection for determination of thiols content in plants tissues of lettuce treated with lead(II) ions (0, 0.5 and 1 mM). We used two HPLC-ED instruments: HPLC coupled with one channel amperometric detector and HPLC coupled with twelve channel coulometric detector to detect simultaneously twelve thiols. The detection limits of thiols measured by CoulArray detector were about two magnitudes lower in comparison to those measured by Coulochem III detector and were from tens to hundreds pM. Under the optimal conditions, we utilized HPLC-CoulArray detector for analysis of tissues from lettuce plants. In addition, distribution and accumulation of lead ions with high spatial resolution was monitored using laser induced breakdown spectroscopy.

• 出版日期2010-6