A Highly Predictive Model for Diagnosis of Colorectal Neoplasms Using Plasma MicroRNA: Improving Specificity and Sensitivity

作者：Carter Jane V; Roberts Henry L; Pan Jianmin; Rice Jonathan D; Burton James F; Galbraith Norman J; Eichenberger Maurice R; Jorden Jeffery; Deveaux Peter; Farmer Russell; Williford Anna; Kanaan Ziad; Rai Shesh N; Galandiuk Susan^*

来源：Annals of Surgery, 2016, 264(4): 575-584.

DOI：10.1097/SLA.0000000000001873

• colorectal adenomas
• colorectal cancer
• diagnostic test
• microRNAs
• plasma test
• screening

Objective:To develop a plasma-based microRNA (miRNA) diagnostic assay specific for colorectal neoplasms, building upon our prior work.Background:Colorectal neoplasms [colorectal cancer (CRC) and colorectal advanced adenoma (CAA)] frequently develop in individuals at ages when other common cancers also occur. Current screening methods lack sensitivity, specificity, and have poor patient compliance.Methods:Plasma was screened for 380 miRNAs using microfluidic array technology from a Training cohort of 60 patients, (10 each) control, CRC, CAA, breast cancer, pancreatic cancer, and lung cancer. We identified uniquely dysregulated miRNAs specific for colorectal neoplasia (P < 0.05, false discovery rate: 5%, adjusted = 0.0038). These miRNAs were evaluated using single assays in a Test cohort of 120 patients. A mathematical model was developed to predict blinded sample identity in a 150 patient Validation cohort using repeat-sub-sampling validation of the testing dataset with 1000 iterations each to assess model detection accuracy.Results:Seven miRNAs (miR-21, miR-29c, miR-122, miR-192, miR-346, miR-372, and miR-374a) were selected based upon P value, area under the curve (AUC), fold change, and biological plausibility. Area under the curve (95% confidence interval) for Test cohort comparisons were 0.91 (0.85-0.96) between all neoplasia and controls, 0.79 (0.70-0.88) between colorectal neoplasia and other cancers, and 0.98 (0.96-1.0) between CRC and colorectal adenomas. In our Validation cohort, our mathematical model predicted blinded sample identity with 69% to 77% accuracy, 67% to 76% accuracy, and 86% to 90% accuracy for each comparison, respectively.Conclusions:Our plasma miRNA assay and prediction model differentiate colorectal neoplasia from patients with other neoplasms and from controls with higher sensitivity and specificity compared with current clinical standards.

• 出版日期2016-10