Automated mems based fruit fly embryo injection system for genome-wide high-throughput RNAi screens

作者：Zappe S^*; Fish M; Scott MP; Solgaard A

来源：8th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2004-09-26 to 2004-09-30.

• fruit fly embryo
• microinjector
• RNA interference
• automated injection
• integrated piezoresistive pressure sensor

We have developed an automated fruit fly embryo injection system for genome-wide RNAi screens. The embryos are attached to glue-covered glass slides, and a MEMS based micro-injector chip is used for injection. Injection procedures are controlled with integrated on-chip piezoresistive pressure sensors. Motorized xyz-stages position the packaged injector chip and the embryo chip relative to each other. Two optical systems with CCD cameras are used for system alignment and embryo positioning. The computer-con trolled setup scans the surface of the glass slide, detects embryos and performs injections. Typically more than 96% of the embryos are reliably injected. A first RNAi experiment is successfully demonstrated.

• 出版日期2005