Amine-Reactive PEGylated Nanoparticles for Potential Bioconjugation

作者：Delaittre Guillaume^*; Justribo Hernandez Gemma; Nolte Roeland J M; Cornelissen Jeroen J L M

来源：Macromolecular Rapid Communications, 2011, 32(1): 19-24.

DOI：10.1002/marc.201000365

• block copolymers
• bioconjugation
• nanoparticles N-succinimidyl ester
• poly(poly(ethylene glycol) methacrylate)

Water-dispersible PEGylated nanoparticles (NPs) presenting amine-reactive conjugation sites at their surfaces were synthesized and their ability to react with amines was demonstrated. An amphiphilic block copolymer bearing an N-succinimidyl ester at its water-soluble end was synthesized by the consecutive controlled radical polymerization of poly(ethylene glycol) methacrylate and styrene from a functional halide initiator. After purification of the copolymer, NPs of approximately 40nm were obtained by a self-assembly process in water. The reactivity of the NPs was evidenced by reacting them with primary amines, including a fluorescent dye. The activated ester remained stable throughout all synthetic steps and a nearly quantitative coupling efficiency was obtained.

• 出版日期2011-1-3