A TaqMan real-time PCR assay for detection of Meloidogyne hapla in root galls and in soil

作者：Sapkota Rumakanta; Skantar Andrea M; Nicolaisen Mogens^*

来源：Nematology, 2016, 18(2): 147-154.

DOI：10.1163/15685411-00002950

• carrot
• Daucus carota
• diagnostics
• ITS
• plant-parasitic nematode
• root-knot nematode

Early detection and quantification of Meloidogyne hapla in soil is essential for effective disease management. The purpose of this study was to develop a real-time PCR assay for detection of M. hapla in soil. Primers and a TaqMan probe were designed for M. hapla detection. The assay detected M. hapla and showed no significant amplification of DNA from non-target nematodes. The assay was able to detect M. hapla in a background of plant and soil DNA. A dilution series of M. hapla eggs in soil showed a high correlation (R-2 = 0.95, P < 0.001) with Ct values. The assay could predict root-knot development in carrots by testing soils before planting. The assay could be useful for management decisions in carrot cultivation.

• 出版日期2016