Differential expression of heat-shock proteins in F2 offspring from F1 hybrids produced between thermally selected and normal rainbow trout strains

作者：Ojima Nobuhiko^*; Mekuchi Miyuki; Ineno Toshinao; Tamaki Koichi; Kera Akio; Kinoshita Shigeharu; Asakawa Shuichi; Watabe Shugo

来源：Fisheries Science, 2012, 78(5): 1051-1057.

DOI：10.1007/s12562-012-0523-3

• Rainbow trout
• Oncorhynchus mykiss
• Thermally selected strain
• Heat-shock proteins
• Gene expression
• Genetic markers
• Western blotting

Rainbow trout Oncorhynchus mykiss is a cold-water aquaculture species, and a thermally selected strain has been developed by multigenerational high-temperature breeding in Japan. We examined the expression of heat-shock proteins as candidates responsible for thermotolerance in rainbow trout using F2 offspring from F1 hybrids produced between thermally selected and normal strains. From F2 offspring, two groups were selected for western blot analysis, namely, low- and high-thermotolerance groups (times to loss of equilibrium were %26lt; 30 and a parts per thousand yen60 min, respectively). We demonstrated that the expression levels of Hsp70, Hsp60, and Hsp40 in tail fin tissues were significantly higher in the individuals with high thermotolerance than in those with low thermotolerance under non-heat-shock conditions. In particular, Hsp70 was expressed only in the individuals with high thermotolerance. These results suggest that Hsp70 is a major protein responsible for conferring thermotolerance in rainbow trout.

• 出版日期2012-9