Nucleosome eviction along with H3K9ac deposition enhances Sox2 binding during human neuroectodermal commitment

作者：Du, Yanhua; Liu, Zhenping; Cao, Xinkai; Chen, Xiaolong; Chen, Zhenyu; Zhang, Xiaobai; Zhang, Xiaoqing^*; Jiang, Cizhong^*

来源：Cell Death and Differentiation, 2017, 24(6): 1121-1131.

DOI：10.1038/cdd.2017.62

• EMBRYONIC STEM-CELLS
• EARLY DROSOPHILA EMBRYOS
• NEURAL DIFFERENTIATION
• TRANSCRIPTION FACTORS
• SUPER-ENHANCERS
• CHROMATIN
• IDENTITY
• VISUALIZATION
• SPECIFICATION
• PLURIPOTENCY

Neuroectoderm is an important neural precursor. However, chromatin remodeling and its epigenetic regulatory roles during the differentiation of human neuroectodermal cells (hNECs) from human embryonic stem cells (hESCs) remain largely unexplored. Here, we obtained hNECs through directed differentiation from hESCs, and determined chromatin states in the two cell types. Upon differentiation, H2A.Z-mediated nucleosome depletion leads to an open chromatin structure in promoters and upregulates expression of neuroectodermal genes. Increase in H3K9ac signals and decrease in H3K27me3 signals in promoters result in an active chromatin state and activate neuroectodermal genes. Conversely, decrease in H3K9ac signals and increase in H3K27me3 signals in promoters repress pluripotency genes. Moreover, H3K9ac signals facilitate the pluripotency factor Sox2 binding to target sites unique to hNECs. Knockdown of the acetyltransferase Kat2b erases H3K9ac signals, disrupts Sox2 binding, and fails the differentiation. Our results demonstrate a hierarchy of epigenetic regulation of gene expression during the differentiation of hNECs from hESCs through chromatin remodeling.

• 出版日期2017-6
• 单位同济大学