A novel multiplex assay of SNP-STR markers for forensic purpose

作者：Wei, Tian; Liao, Fei; Wang, Yaowu; Pan, Chao; Xiao, Chao; Huang, Daixin^*

来源：PLos One, 2018, 13(7): e0200700.

DOI：10.1371/journal.pone.0200700

• UNBALANCED DNA MIXTURES
• SHORT TANDEM REPEATS
• SEXUAL ASSAULT
• DIP-STR
• LASER MICRODISSECTION
• EPITHELIAL-CELLS
• SPERM CELLS
• DIFFERENTIAL EXTRACTION
• SIZE DISTRIBUTIONS
• FLOW-CYTOMETRY

Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase chain reaction (PCR) assay was developed for amplifying these SNP-STRs and the Amelogenin gender marker according to the principle of amplification refractory mutation system (ARMS). This novel multiplex set allows detection of the minor DNA contributor in a DNA mixture of any gender and cellular origin with high resolution (beyond a DNA ratio of 1:20). In addition, SNP-STR haplotype frequencies were estimated based on a survey of 350 unrelated individuals from Chinese Han population, and the combined power of discrimination (PD) and power of exclusion (PE) of the eight SNP-STRs were calculated as 0.99999999965 and 0.9996, which were obviously higher than that of the eight STR loci: 0.9999999954 and 0.9989 respectively. The results indicated that the SNP-STR compound markers have higher application value in forensic identification compared to standard autosomal STRs, especially in the analysis of imbalanced DNA mixtures.

• 出版日期2018-7-18
• 单位华中科技大学