Aquimarina pacifica sp nov., isolated from seawater

作者：Zhang Zenghu; Yu Tong; Xu Tingting; Zhang Xiao Hua^*

来源：International Journal of Systematic and Evolutionary Microbiology, 2014, 64(Pt 6): 1991-1997.

DOI：10.1099/ijs.0.062695-0

A Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic bacterium with gliding motility, designated strain SW150(T), was isolated from surface seawater of the South Pacific Gyre (39 degrees 19'S 139 degrees 48' W) during the Integrated Ocean Drilling Program Expedition 329. Optimal growth occurred in the presence of 2-4% (w/v) NaCl, at pH 7-8 and at 28-30 degrees C. The dominant fatty acids were iso-C-15:0, iSo-C-17:0 3-OH, iso-C-15:1 G, C-16:1 omega 6c and/or C-16:1 omega 7c and 10-methyl C-16:0 and/or iso-C-17:1 omega 9c. The polar lipids of strain SW150(T) comprised phosphatidylethanolamine, three unknown polar lipids and one unknown aminolipid. The major respiratory quinone was MK-6. The DNA G+C content of strain SW150(T) was 33.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina macrocephali JAMB N27(T) and Aquimarina muelleri KMM 6020(T) with 97.8 and 96.8% sequence similarities, respectively. The estimated DNA-DNA hybridization values were 21.00 +/- 2.33% between strain SW150(T) and A. macrocephali JAMB N27(T) and 20.60 +/- 2.32% between strain SW150(T) and Aquimarina megaterium XH134(T). On the basis of polyphasic analyses, strain SW 150(T) represents a novel species of the genus Aquimarina, for which the name Aquimarina pacifica sp. nov. is proposed. The type strain is SW150(T) (=JCM 18214(T)=CGMCC 1.12180(T)).

• 出版日期2014-6
• 单位中国海洋大学