Graphene-based lysozyme binding aptamer nanocomposite for label-free and sensitive lysozyme sensing

作者：Xiao, Yinghong^*; Wang, Yanping; Wu, Min; Ma, Xiaoling; Yang, Xiaodi

来源：Journal of Electroanalytical Chemistry, 2013, 702: 49-55.

DOI：10.1016/j.jelechem.2013.05.010

• Graphene
• Aptasensor
• Functionalized electrode
• Nanocomposite sensing platform

This work presents a facile route to fabricate a highly sensitive platform for the recognition of lysozyme. Graphene oxide was coated on the surface of a glassy carbon electrode (GCE) followed by reduction with hydrazine to obtain a graphene (GR) coated GCE. After activation of GR-GCE with EDC and NHS, lysozyme binding aptamer (LBA) was molecularly tethered onto GR to obtain a highly sensitive platform named as LBA-GR-GCE. Results show that GR greatly promotes the electron transfer at electrode/electrolyte interface due to its excellent electrical conductivity. LBA-GR-GCE is able to quantitatively detect the analyte of lysozyme in real samples of human saliva and chicken egg white with high selectivity, with a linear range of 0.01-0.5 pmol L-1 and detection limit of 6 fmol L-1 (S/N = 3). Furthermore, the surface feature of the functionalized electrode was investigated to determine its effect on charge transfer resistance with two different electrochemical probes of [Fe(CN)(6)](3-/4-) and Ru(NH3)(6)(3+).

• 出版日期2013-8-1
• 单位南京师范大学; 南京医科大学