Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression

作者：Mao, Jie-Li; Miao, Zi-Qing; Wang, Zhen; Yu, Lin-Hui; Cai, Xiao-Teng; Xiang, Cheng-Bin^*

来源：PLoS Genetics, 2016, 12(1): e1005760.

DOI：10.1371/journal.pgen.1005760

• RESPONSIVE GENE-EXPRESSION
• DNA-BINDING DOMAIN
• SIGNAL-TRANSDUCTION
• TRANSCRIPTION FACTORS
• INSENSITIVE MUTANTS
• NECROTROPHIC FUNGI
• PLANT DEVELOPMENT
• STRESS RESPONSES
• CELL ELONGATION
• LOW-TEMPERATURE

The gaseous phytohormone ethylene participates in the regulation of root growth and development in Arabidopsis. It is known that root growth inhibition by ethylene involves auxin, which is partially mediated by the action of the WEAK ETHYLENE INSENSITIVE2/ANTHRA NILATE SYNTHASE alpha 1 (WEI2/ASA1), encoding a rate-limiting enzyme in tryptophan (Trp) biosynthesis, from which auxin is derived. However, the molecular mechanism by which ethylene decreases root growth via ASA1 is not understood. Here we report that the ethylene-responsive AP2 transcription factor, ETHYLENE RESPONSE FACTOR1 (ERF1), plays an important role in primary root elongation of Arabidopsis. Using loss-and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth. This discloses one mechanism linking ethylene signaling and auxin biosynthesis in Arabidopsis roots.

• 出版日期2016-1
• 单位中国科学技术大学