Analytical Method for Pesticides in Avocado and Papaya by Means of Ultra-High Performance Liquid Chromatography Coupled to a Triple Quadrupole Mass Detector: Validation and Uncertainty Assessment

作者：Pano Farias Norma S; Ceballos Magana Silvia G; Jurado Jose M; Aguayo Villarreal Ismael A; Muniz Valencia Roberto^*

来源：Journal of Food Science, 2018, 83(8): 2265-2272.

DOI：10.1111/1750-3841.14210

• avocado
• matrix effect
• papaya
• pesticides
• uncertainty

In this work, the uncertainty estimation for the determination of ametryn, carbofuran, atrazine, carbaryl, and methyl parathion in papaya and avocado is presented, along with other validation parameters. The analytical method was developed using Quick, Easy, Cheap, Effective, Rugged, and Safe extraction and ultrahigh performance liquid chromatography coupled to a triple quadrupole mass detector. The method validation showed that the linear correlation coefficients were higher than 0.99 for both fruits. The limits of detection for avocado and papaya were in the range of 0.022 to 0.46 and 0.003 to 0.109 g/g, respectively. Intermediate precision varied from 5.3% to 13.0% in papaya, and from 4.8% to 20.2% in avocado. Recoveries obtained for each pesticide in both matrices ranged between 61.3% and 119.0%. Matrix effect was calculated for all compounds in both fruits. Finally, the overall uncertainty was lower than 36% for both fruits.
Practical ApplicationThe present analytical method could be used for pesticides determination in different kind of fruits as papaya and avocado and as a practical guide for uncertainty and matrix effect determination.

• 出版日期2018-8