A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum

作者：Wu, Lichang; Sun, Yu; Ma, Liqiao; Zhu, Jun; Zhang, Baoxia; Pan, Qingjie; Li, Yuyin; Liu, Huanqi; Diao, Aipo^*; Li, Yinchuan^*

来源：Scientific Reports, 2016, 6(1): 27332.

DOI：10.1038/srep27332

• BESTROPHIN FAMILY
• CA2+ HOMEOSTASIS
• TUMOR PROMOTER
• CALCIUM
• CHANNEL
• IDENTIFICATION
• RELEASE
• PROTEIN
• FUSION
• GENE

The Bestrophin family has been characterized as Cl-channels in mammals and Na+ channels in bacteria, but their exact physiological roles remian unknown. In this study, a natural C-terminally truncated variant of mouse Bestrophin 3 (Best3V2) expression in myoblasts and muscles is demonstrated. Unlike full-length Best3, Best3V2 targets the two important intracellular Ca stores: the lysosome and the ER. Heterologous overexpression leads to lysosome swelling and renders it less acidic. Best3V2 overexpression also results in compromised Ca2+ release from the ER. Knocking down endogenous Best3 expression in myoblasts makes these cells more excitable in response to Ca2+ mobilizing reagents, such as caffeine. We propose that Best3V2 in myoblasts may work as a tuner to control Ca2+ release from intracellular Ca2+ stores.

• 出版日期2016-6-6
• 单位天津科技大学; 青岛农业大学