Atmospheric pressure visible-wavelength MALDI-MS

作者：Sun Zhen; Findsen Eric W; Isailovic Dragan^*

来源：International Journal of Mass Spectrometry, 2012, 315: 66-73.

DOI：10.1016/j.ijms.2012.03.002

• Atmospheric pressure
• Visible-wavelength MALDI-MS
• Dye
• Peptide
• Oligosaccharide
• Synthetic polymer

An atmospheric pressure (AP) visible-wavelength MALDI source was developed and coupled to a quadrupole time-of-flight (Q-TOF) mass spectrometer. This instrument employed a pulsed laser emitting light at a wavelength of 532 nm to desorb and ionize samples such as dyes, oligosaccharides, peptides, and synthetic polymers at atmospheric pressure. Ions formed were analyzed by the Q-TOF in both MS and tandem MS (MS/MS) modes. Several visible-wavelength absorbing dyes were successfully utilized and studied as matrices. Among these dyes, nuclear fast red (NFR), rhodamine B isothiocyanate (RITC), and 5(6)-carboxytetramethyl-rhodamine N-succinimidyl ester (TAMRA, SE) are reported as effective visible-wavelength MALDI-MS matrices for the first time. This report demonstrates applicability of visible-wavelength AP MALDI-MS and AP MALDI MS/MS to the detection and structural analysis of dye molecules, biomolecules, and synthetic polymers.

• 出版日期2012-4-1