A NEW MECHANISM FOR COACTIVATION OF TRANSCRIPTION INITIATION - REPOSITIONING OF AN ACTIVATOR TRIGGERED BY THE BINDING OF A 2ND ACTIVATOR

作者：RICHET E^*; VIDALINGIGLIARDI D; RAIBAUD O

来源：Cell, 1991, 66(6): 1185-1195.

DOI：10.1016/0092-8674(91)90041-V

The cAMP receptor protein (CRP) and MalT, the maltose regulon activator, synergistically activate transcription from the E. coli malKp promoter. The malKp regulatory region comprises two series of MalT-binding sites separated by three CRP-binding sites. By combining genetic and biochemical studies, we demonstrate that the promoter-proximal region contains two overlapping sets of three MalT-binding sites. Occupation of the higher affinity set of sites, which occurs in the absence of CRP, does not lead to malKp activation. In contrast, in the presence of CRP, MalT binds to the lower affinity set of sites and triggers transcription initiation because, unlike the high affinity set, the low affinity set of sites is properly positioned with respect to the Pribnow box. The CRP effect requires the malKp-distal MalT-binding sites. The synergistic action of MalT and CRP therefore relies on MalT repositioning via the formation of a nucleoprotein structure involving the entire regulatory region.

• 出版日期1991-9-20