Multiphoton Microscopic Imaging of Normal Human Rectum Tissue

作者：Liu, Nenrong; Chen, Gang; Chen, Jianxin^*; Yan, Jun; Zhuo, Shuangmu; Zheng, Liqin; Jiang, Xingshan

来源：Scanning, 2010, 32(6): 347-350.

DOI：10.1002/sca.20194

• multiphoton microscopy (MPM)
• second harmonic generation (SHG)
• two-photon excited fluorescence (TPEF)
• imaging
• rectum tissue
• histology

In this paper, multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation signals, was used to image microstructures of human rectal mucosa and submucosa. The morphology and distribution of the main components in mucosa layer, goblet cells, intestinal glands, and a little collagen fibers have been clearly monitored, and the content and distribution of collagen, elastic fibers, and blood vessels in submucosa layer have also been distinctly obtained. The variation of these components is very relevant to the pathology in gastrointestinal system, especially early rectal cancer. Our results indicate that the MPM technique has the potential application in vivo in the clinical diagnosis and monitoring of early rectal cancer. SCANNING 32: 347-350, 2010.

• 出版日期2010-12
• 单位福建师范大学; 福建省肿瘤医院