Systems biotechnology of animal cells: the road to prediction

作者：Carinhas Nuno; Oliveira Rui; Alves Paula Marques; Carrondo Manuel J T; Teixeira Ana P^*

来源：Trends in Biotechnology, 2012, 30(7): 377-385.

DOI：10.1016/j.tibtech.2012.03.004

• systems biotechnology
• mathematical modeling
• computational prediction
• animal cell culture
• recombinant protein
• bioprocess improvement

A central concern of biopharmaceutical R&D is the production of sufficient quantities of recombinant products from manufacturing processes based on animal cell culture. The way in which bioprocess researchers have addressed this question experienced a tremendous shift over the years, progressing from almost empirical to more rational approaches. A step further is the application of systems biotechnology: recent technological advances for large-scale cell state characterization and creative methods for host cell modeling are becoming crucial for next-generation bioprocess optimization. Here we provide an overview of the main trends towards this goal, with a focus on metabolic models as central scaffolds for data integration and prediction of bioprocess outcomes.

• 出版日期2012-7