科研之友
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摘要
Objective: The impaired diabetic wound healing represents a significant clinical problem. Human stromal vascular fraction cells (SVFs) have been recognized as an attractive stem cell source. The aim of this study was to investigate the efficacy of adipose-derived SVFs with microneedles injection technology on diabetic wound healing. Methods: The diabetic porcine models were established on 4 female domestic pigs through injection of streptozocin (STZ) with their skin on the back being removed. The diabetic porcine pigs were randomly divided into 4 groups, i.e. PBS group (n = 12), HA group (n = 12), SVFs group (n = 12) and HA+SVFs group (n = 12). Thereafter, treatment with the corresponding drugs was carried out and the healing rate in each group was recorded and compared among groups. In addition, we also collected the tissue samples to perform histological analysis. Results: In HA+SVFs group, we found that wound healing rate was significantly accelerated in comparison with other three groups, and the HA and SVFs also have positive effect individually on wound healing. HA+SVFs had highest capillary density (31.83 +/- 3.19) compared with experimental groups (14.67 +/- 1.86, 21.83 +/- 2.56) and control group (8.16 +/- 1.17). The higher protein expression of VEGF (295.52 +/- 49.21 pg/ml) and bFGF (21.07 +/- 3.47 pg/ml) in group HA+SVFs was found than other groups. Conclusions: Local injection of HA+SVFs with microneedles injection technology can significantly accelerate the healing rates of wound on porcine models, and this may be an alternative method for treatment of wounds in diabetic patients.
