科研之友
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摘要
Previous studies have demonstrated an association between neurological diseases and oxidative stress (OS). Naphthoflavone is a synthetic derivative of naturally occurring flavonoids that serves an important role in the treatment and prevention of OS-related diseases. The current study was designed to apply alpha- and beta-Naphthoflavone individually and in combination to counteract the detrimental effects of OS on neurons in vitro. Neuronal SH-SY5Y cells were subjected to 20 mu M H2O2, followed by exposure to 20 mu M alpha-Naphthoflavone and/or 10 mu M beta-Naphthoflavone. Results indicated that alpha- and beta-Naphthoflavone effectively antagonized the apoptosis-promoting effect of H2O2 on neuronal SH-SY5Y cells, and that beta-Naphthoflavone significantly (P<0.05) reversed H2O2-inhibited cell viability. Notably, co-treatment of alpha- and beta-Naphthoflavone reversed the H2O2-induced apoptosis rate elevation and cell viability reduction. Further analysis demonstrated that H2O2 inhibited the activities of antioxidant enzymes including catalase, superoxide dismutase and glutathione peroxidase, but this was reversed by the co-treatment with alpha- and beta-Naphthoflavone and selectively enhanced by the treatment with alpha- or beta-Naphthoflavone. H2O2-stimulated p38 mitogen-activated protein kinase activation was repressed following treatment with alpha- and/ or beta-Naphthoflavone, along with a decreased expression of the apoptosis-related factors and inhibited caspase-3 activation. In conclusion, co-treatment with alpha- and beta-Naphthoflavone minimized H2O2-led neuron damage compared with treatment with alpha- or beta-Naphthoflavone, suggesting a synergetic effect between alpha- and beta-Naphthoflavone. This indicates that utilizing alpha- and beta-Naphthoflavone together in the clinical setting may provide a novel therapeutic for neurological disease.
- 出版日期2017-3
- 单位湖南大学
