A B-ARR-mediated cytokinin transcriptional network directs hormone cross-regulation and shoot development

作者：Xie, Mingtang; Chen, Hongyu; Huang, Ling; O'Neil, Ryan C.; Shokhirev, Maxim N.; Ecker, Joseph R.^*

来源：Nature Communications, 2018, 9(1): 1604.

DOI：10.1038/s41467-018-03921-6

• ARABIDOPSIS RESPONSE REGULATORS
• STEM-CELL NUMBER
• SIGNAL-TRANSDUCTION
• PLANT DEVELOPMENT
• ABSCISIC-ACID
• RNA-SEQ
• WUSCHEL
• GENES
• PROTEINS
• IDENTIFICATION

Cytokinin fulfills its diverse roles in planta through a series of transcriptional responses. We identify the in vivo DNA binding site profiles for three genetically redundant type-B ARA-BIDOPSIS RESPONSE REGULATORS (B-ARRs): ARR1, ARR10, and ARR12. The expression and genome-wide DNA binding locations of the three B-ARRs extensively overlap. Constructing a primary cytokinin response transcriptional network reveals a recurring theme of widespread cross-regulation between the components of the cytokinin pathway and other plant hormone pathways. The B-ARRs are found to have similar DNA binding motifs, though sequences flanking the core motif were degenerate. Cytokinin treatments amalgamate the three different B-ARRs motifs to identical DNA binding signatures (AGATHY, H(a/t/c), Y(t/c)) which suggests cytokinin may regulate binding activity of B-ARR family members. Furthermore, we find that WUSCHEL, a key gene required for apical meristem maintenance, is a cytokinin-dependent B-ARR target gene, demonstrating the importance of the cytokinin transcription factor network in shoot development.

• 出版日期2018-4-23