Anti-depressive effect of polyphenols and omega-3 fatty acid from pomegranate peel and flax seed in mice exposed to chronic mild stress

作者：Naveen Shivanna^*; Siddalingaswamy Mahadevappa; Singsit Dongzagin; Khanum Farhath

来源：Psychiatry and Clinical Neurosciences, 2013, 67(7): 501-508.

DOI：10.1111/pcn.12100

• CMS
• flax seed
• neurotransmitters
• pomegranate peel
• sucrose

AimIn this study polyphenols from pomegranate peel, and n-3 fatty acids with polyphenols from flax seed were evaluated for their anti depression properties in mice exposed to chronic mild stress (CMS).
MethodsA total of 40 mice initially trained to consume 2% sucrose solution for 3 weeks were then divided into five groups of eight each. The first group was the normal control, the remaining four groups were exposed to CMS but were force fed with either: 10mL water per kg bodyweight per day; imipramine (a standard antidepressant) 15mg kg bodyweight; 30mg per kg bodyweight polyphenol equivalent extract from pomegranate peel; or 30mg polyphenols per kg bodyweight with omega-3 fatty acids present, for 50days. At the end, blood and brain were analyzed for various biomarkers of depression.
ResultsThe flax seed and imipramine groups had significantly increased sucrose consumption, decreased cortisol (blood), decreased epinephrine and norepinephrine concentration, decreased monoamine oxidase A and B activity, and decreased superoxide dismutase activity. Lipid peroxidation was completely inhibited. In contrast, pomegranate peel extract also completely inhibited lipid peroxidation in the brain, and reduced enzyme activity and hormone concentration but to a lesser extent than flax seed.
ConclusionPolyphenols from flax seed with omega-3 fatty acids were able to reduce all the CMS effects tested compared to polyphenols from pomegranate peel.

• 出版日期2013-11