An ultraperformance liquid chromatography method for the normal-phase separation of lipids

作者：McLaren David G^*; Miller Paul L; Lassman Michael E; Castro Perez Jose M; Hubbard Brian K; Roddy Thomas P

来源：Analytical Biochemistry, 2011, 414(2): 266-272.

DOI：10.1016/j.ab.2011.03.009

• Lipids
• Ultraperformance liquid chromatography
• Evaporative light-scattering detection
• High throughput
• Normal-phase chromatography

An ultraperformance liquid chromatography method using normal-phase solvents, a silica column, and evaporative light-scattering detection is presented. The method is based on a quaternary gradient profile and is capable of resolving the major neutral and polar lipids present in plasma and animal tissue in under 5 min, with a total cycle time of 11 min. Limits of quantitation for 7 different lipid classes were on the order of 200 ng of material on column which enables an accurate analysis from as little as 20 mu L of plasma or 50 mg of tissue for typical samples. Intraday and interday precision for the determination of the major lipid classes in human plasma ranged from 3.6 to 10.5% CV with a variability in retention time of less than 6%. The utility of the method is demonstrated through the separation and quantitation of lipids in mouse plasma, liver, and heart tissue.

• 出版日期2011-7-15