Application of First Order Derivative UV Spectrophotometry Coupled with H-Point Standard Addition to the Simultaneous Determination of Melamine and Dicyandiamide in Milk

作者：Zou, Huayu; Xu, Kailin; Feng, Yuyan; Liang, Bing^*

来源：Food Analytical Methods, 2015, 8(3): 740-748.

DOI：10.1007/s12161-014-9938-z

• Melamine
• Dicyandiamide
• Milk
• Simultaneous determination
• HPSAM

A simple and rapid method for simultaneous determination of melamine and dicyandiamide in milk was developed, in which first order derivative UV spectrophotometry was coupled with the improved H-point standard addition proposed in our previous paper for melamine and with K-ratio-H-point standard addition for dicyandiamide, respectively. Dicyandiamide and melamine did not influence with each other determination. The method was applied successfully to the determination of melamine and dicyandiamide in three kinds of milk samples, including two kinds of milk powder and one kind of liquid milk. The relative standard deviation (n = 3) for the determination of melamine was in the range of 4.37 similar to 6.05 % with recoveries of 94.3 similar to 110.6 %, for dicyandiamide was in the range of 0.12 similar to 3.44 % with recoveries of 94.27 similar to 110.6 %. The proposed method can be applied to samples containing melamine higher than 0.69 mg kg(-1) in milk powder and 0.15 mg L-1 in liquid milk, and dicyandiamide higher than 0.011 mg kg(-1) in milk powder and 0.013 mg L-1 in liquid milk. Furthermore, it is organic solvents-free and less laborious.

• 出版日期2015-3
• 单位四川大学