Anthocyanin content in rice is related to expression levels of anthocyanin biosynthetic genes

作者：Kim Bong Gyu; Kim Jeong Ho; Min Shin Young; Shin Kwang Hee; Kim Ji Hye; Kim Hong Yeol; Ryu Su Noh; Ahn Joong Hoon^*

来源：Journal of Plant Biology, 2007, 50(2): 156-160.

DOI：10.1007/bf03030624

• anthocyanin biosynthesis
• Oryza sativa
• real-time PCR

The composition of anthocyanins was analyzed in two rice cultivars. 'Ilpum' showed no detectable levels, while 'Heugjinju' contained three types of anthocyanins: cyanidin, cyanidin 3-O-glucoside, and peonidin 3-O-glucoside. We also assessed the expression of anthocyanin biosynthetic genes - for phenylallanine ammonia lyase (PAL), chalcone synthase (CHS), flavanone 3 beta-hydroxylase (F3H), dihydroflavonol reductase (DFR), and anthocyanin synthase (ANS) - in different tissues of those cultivars. All five genes were expressed more in the leaves and seeds of 'Heugjinju' than in Ilpum', with the greatest amount of transcript being detected in 'Heugjinju' seeds. Two genes, DFR and ANS, had relatively high expression levels and were specific for anthocyanin biosynthesis. Furthermore, expression of CHS, F3H, DFR, and ANS was enhanced during seed maturation and was correlated with ambient temperature during seedling growth.

• 出版日期2007-4-30