The Relationship Between NO, ADMA and Homocysteine in Endotoxin-Mediated Inflammation in HUVEC Cultures

作者：Pasaoglu Ozge Tugce^*; Turkozkan Nurten; Ark Mustafa; Polat Belgin; Agilli Mehmet; Yaman Halil

来源：Turkish Journal of Biochemistry, 2013, 38(3): 258-261.

DOI：10.5505/tjb.2013.47966

• nitric oxide
• ADMA
• homocysteine
• HUVEC
• endotoxemia

Objective: The aim of our study was to investigate the relationship between Nitric Oxide, ADMA, homocysteine in endotoxin-induced human umblical vein endothelial cell cultures. %26lt;br%26gt;Methods: For this reason two groups are formed (n=12). Control group consists of HUVEC cultures without any treatment. LPS group is treated with 10 mu g/mL endotoxin (E.coli 0111: B4). The measurement of NO levels is perfomed by spectrophotometric measurement of nitrite and nitrate. ADMA and Homocysteine levels are measured using HPLC with fluorescence detection. %26lt;br%26gt;Results: The results of our study showed that there was a significant increase of NO, ADMA and Homocysteine in endotoxemia (p%26lt;0.05). %26lt;br%26gt;Conclusion: In our inflammation model, both Arginine-ADMA and Arginine-NO pathways are found to be activated. In this model, the NOS isoform catalysed NO synthesis is iNOS. We speculate ADMA, which was formed as a result of LPS stimulation, was not sufficient to inhibit iNOS and it also caused eNOS to uncouple and contributed to peroxynitrite formation, thus lead to a dramatic increase in nitrate/nitrite levels. Furthermore, increased oxidative stress caused by elevated homocysteine levels might have contributed to increased NOx levels via iNOS activation and ADMA accumulation. As a result, endothelial dyfunction that may arise from the relationship between NO-ADMA-Homocysteine might provide a different point of view to atherosclerotic diseases.

• 出版日期2013