Antimicrobial efficacy of an innovative emulsion of medium chain triglycerides against canine and feline periodontopathogens

作者：Laverty G^*; Gilmore B F; Jones D S; Coyle L; Folan M; Breathnach R

来源：Journal of Small Animal Practice, 2015, 56(4): 253-263.

DOI：10.1111/jsap.12344

OBJECTIVESTo test the in vitro antimicrobial efficacy of a non-toxic emulsion of free fatty acids against clinically relevant canine and feline periodontopathogens
METHODSAntimicrobial kill kinetics were established utilising an alamarBlue((R)) viability assay against 10 species of canine and feline periodontopathogens in the biofilm mode of growth at a concentration of 0125% v/v medium chain triglyceride (ML:8) emulsion. The results were compared with 012% v/v chlorhexidine digluconate and a xylitol-containing dental formulation. Mammalian cellular cytotoxicity was also investigated for both the ML:8 emulsion and chlorhexidine digluconate (025 to 00625% v/v) using in vitro tissue culture techniques.
RESULTSNo statistically significant difference was observed in the antimicrobial activity of the ML:8 emulsion and chlorhexidine digluconate; a high percentage kill rate (>70%) was achieved within 5 minutes of exposure and was maintained at subsequent time points. A statistically significant improvement in antibiofilm activity was observed with the ML:8 emulsion compared with the xylitol-containing formulation. The ML:8 emulsion possessed a significantly lower (P < 0001) toxicity profile compared with the chlorhexidine digluconate in mammalian cellular cytotoxicity assays.
CLINICAL SIGNIFICANCEThe ML:8 emulsion exhibited significant potential as a putative effective antimicrobial alternative to chlorhexidine- and xylitol- based products for the reduction of canine and feline periodontopathogens.

• 出版日期2015-4