Hydrogen peroxide biosensor based on the direct electrochemistry of myoglobin immobilized on silver nanoparticles doped carbon nanotubes film

作者：Liu Chuan Yin; Hu Ji Ming^*

来源：Biosensors and Bioelectronics, 2009, 24(7): 2149-2154.

DOI：10.1016/j.bios.2008.11.007

• Hydrogen peroxide
• Myoglobin
• Biosensor
• Silver nanoparticles doped carbon nanotubes

A novel H(2)O(2) biosensor has been fabricated based on the direct electrochemistry and electrocatalysis of myoglobin (Mb) immobilized on silver nanoparticles doped carbon nanotubes film with hybrid sol-gel techniques. A pair of redox peaks with peak separation of 160 mV and formal potential of -0.295 V was observed at this composite film, corresponding to the direct electrochemistry of Mb. The heterogeneous rate constant was estimated to be 0.41 s(-1). Under optimum conditions, the amperometric determination of H(2)O(2) was performed with a linear range of 2.0 x 10(-6) -1.2 x 10(-3) mol L(-1) and a detection limit of 3.6 x 10(-7) mol/L (S/N = 3). The Michealis-Menten constant was also estimated to be 1.62 mmol L(-1). The proposed biosensor showed favorable reproducibility, stability, selectivity and accuracy, and has been used to determine H(2)O(2) in real samples with favorable recoveries.

• 出版日期2009-3-15
• 单位南宁师范大学; 武汉大学