Detection of proteins on poly(vinylidene difluoride) membranes by scanning electrochemical microscopy

作者：Carano M; Lion N; Abid JP; Girault HH^*

来源：Electrochemistry Communications, 2004, 6(12): 1217-1221.

DOI：10.1016/j.elecom.2004.09.018

• protein detection
• scanning electrochemical microscopy
• SECM
• silver nanoparticles
• polyvinylidene difluoride
• PVDF membrane

Scanning electrochemical microscopy (SECM) is used in combination with silver nanoparticles tagging to achieve visualization of proteins on surfaces. Proteins are adsorbed on a polyvinylidene difluoride (PVDF) membrane, tagged with silver nanoparticles and then detected by SECM with osmium tris-bipyridine as a redox mediator in aqueous solution. During the SECM scan, the potential is held at a value at which the oxidation of the redox mediator occurs and a positive feedback current is detected when scanning over silver nanoparticles. A negative feedback is observed elsewhere. This method enables protein adsorption mapping on polymeric membranes.

• 出版日期2004-12