Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis

作者：Young Chao Wang; Hsieh Jia Ling; Ay Chyung^*

来源：Sensors (Switzerland), 2012, 12(3): 2400-2413.

DOI：10.3390/s120302400

• electroosmotic flow
• cell lysis
• dielectrophoresis force
• cell manipulation

This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 mu m/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.

• 出版日期2012-3