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摘要
To explore the effects of tumor necrosis factor-alpha (TNF-alpha) on ventricular arrhythmias (VAs) in rats with acute myocardial infarction (AMI). A rat model of AMI was created by ligation of the left anterior descending (LAD) coronary artery. ECG and spontaneous VAs were observed during the whole experiment. Monophasic action potentials (MAP) among ischemic zone, border zone and non-ischemia zone was observed and Monophasic action potential repolarization dispersion (MAPDd) were calculated. Linear correlation analysis was used to assess the relationship between TNF-alpha protein expression and MAPDd of the border zone. The incidence of VAs in AMI group was markedly increased at each time interval after ligation compared with Etanercept group (P<0.05). The MAPDd from the border zone of the ischemic myocardium was significantly increased at each time point after ligation compared with the ischemic zone and non-ischemic zone of the epicardium (P<0.05). Moreover, the MAPDd from the border zone of ischemic myocardium was significantly increased in the AMI group compared with the Etanercept group (P<0.05). The temporal changes of the MAPDd in the border zone coincided with the incidence of VAs in vivo during early AMI. The levels of TNF-alpha protein expression in the AMI group began to increase at 10 min and reached a peak at 20 min after ligation; then, they decreased. The correlation coefficient of TNF-alpha protein expression and MAPDd of the border zone was 0.96 (P<0.01) and showed a positive linear correlation. TNF-alpha could increase the MAPDd in the border zone, and promote the onset of VAs, while Etanercept could decrease the MAPDd in the border zone and lessen the incidence of VAs in rats with AMI.
- 出版日期2016
- 单位郑州大学
