科研之友
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摘要
Electrocatalysis of redox enzymes shows wide application for biosensing. DNAzymes exhibiting specific catalytic activities have aroused great interest recently. However, there are few studies on the electrocatalysis between DNAzyme and electron mediator. In this paper, based on the electrocatalysis of methylene blue (MB) and horseradish peroxidase mimicking DNAzyme (HRP-DNAzyme), an amplified electrochemical biosensor for the detection of adenosine triphosphate (ATP) was designed. In the present system, by means of the ATP-aptamer interaction, two guanine-rich DNA sequences, one of which was labeled with MB at the 5 end, were assembled on the gold electrode. In the presence of K+ and hemin, the guanine-rich DNA sequences transferred to HRP-DNAzyme. The conformational change of the structure resulted in the approaching of MB and HRP-DNAzyme which made the electrocatalytic process between MB and HRP-DNAzyme possible. We used cyclic voltammetry and electrochemical impedance spectroscopy to study the electrocatalytic process. The system was therefore utilized for amplified detection of ATP without imposing any new constraints to the platform which showed satisfactory result.
- 出版日期2014-2
- 单位安徽师范大学
