Scanning Electrochemical Microscopy for Electrochemical Detection of Single-base Mismatches by Tagging Ferrocenecarboxylic Acid as a Redox Probe to DNA

作者：Moradi Nasrin; Noori Abolhassan; Mehrgardi Masoud A; Mousavi Mir F

来源：Electroanalysis, 2016, 28(4): 823-832.

DOI：10.1002/elan.201500598

• Single-base mismatch (SBM)
• Ferrocenecarboxylic acid
• Scanning electrochemical microscopy
• DNA-mediated charge transfer
• Bimolecular electron transfer

Scanning electrochemical microscopy (SECM) was employed for sensitive detection of single base mismatches (SBMs) in a sandwiched dsDNA. Ferrocenecarboxylic acid (Fc), covalently conjugated to the dsDNA, was oxidized to Fc(+) via the DNA-mediated charge transfer from the underlying gold susbtrate, and reduced back to Fc by SECM tip generated ferrocyanide. The electrocatalytic oxidation of SECM tip-generated ferrocyanide was sensitive to presence, as well as the type of SBMs. Apparent standard rate constants (k(app)(0)) values for different SBMs, both near the electrode surface and far from it, were evaluated by SECM. The method can detect SBMs independent of their position in dsDNA.

• 出版日期2016-4