Mouse ovarian-related gene expression profiles change with intraperitoneal injection of cisplatin

作者：Ai Hao^*; Zhang Haiying; Zhang Yun; Liu Yang

来源：African Journal of Pharmacy and Pharmacology, 2012, 6(28): 2119-2122.

DOI：10.5897/AJPP12.272

• Ovary
• cisplatin
• gene expression pattern
• nucleotide sequence analysis
• microarray hybridization

Cisplatin (DDP), an important anti-cancer drug, can affect normal ovarian function. However, the underlying pathophysiological mechanism is unclear. Thus, to analyze the mechanism of its ovary function, modulation effect was evaluated by noting the effect of its injection on ovary related gene expression in mice. Total mRNA of mice ovary tissue after administration of cisplatin and the normal control group was extracted by TRIzol method. After reverse transcription and fluorescent labeling by Cy3, Cy5, we obtained two groups of mouse ovarian cDNA probes and then hybridized with the cDNA gene expression profiles microarray hybridization. The cDNA microarray was scanned using the fluorescent signals and showed differences between two groups with the help of statistical analysis program. By analyzing the ovarian tissue gene expressions after the administration of cisplatin, 109 differentially expressed genes were identified, among which were 19 up-regulated expression genes and 90 down-regulated expression genes. These genes may participate in the occurrence and development of ovary dysfunction induced by cisplatin and provides diagnosis evidence for ovary dysfunction induced by chemotherapy.

• 出版日期2012-7
• 单位辽宁大学