AKAP2 is upregulated in ovarian cancer, and promotes growth and migration of cancer cells

作者：Li, Xin; Wang, Changjun; Zhang, Gang; Liang, Ming; Zhang, Bin^*

来源：Molecular Medicine Reports, 2017, 16(4): 5151-5156.

DOI：10.3892/mmr.2017.7286

• A-kinase anchoring protein 2
• ovarian cancer
• beta-catenin/T cell factor signaling
• cell growth
• cell migration

Mutations of A-kinase anchoring protein 2 (AKAP2) have been reported to be associated with adolescent idiopathic scoliosis. However, its role in cancer is poorly understood. In the present study, the mRNA levels of AKAP2 in ovarian cancer tissues were examined using qPCR. The effects of AKAP2 on the growth and migration of cancer cells were examined using crystal violet and Boyden chamber assays. An in vivo image system was used to evaluate the effect of AKAP2 on the metastasis of ovarian cancer cells. The present study demonstrated that the expression of AKAP2 was elevated in ovarian cancer. Furthermore, overexpression of AKAP2 promoted the growth and migration of ovarian cancer cells, whereas knockdown of AKAP2 expression reduced the growth and migration of ovarian cancer cells. Analysis of the molecular mechanism indicated that AKAP2 activated beta-catenin/T cell factor signaling and regulated the expression of several target genes. Furthermore, analysis of the in vivo metastatic capacity demonstrated that downregulation of AKAP2 inhibited the invasion of ovarian cancer cells. Taken together, the present study demonstrated an oncogenic role for AKAP2 in ovarian cancer, indicating that AKAP2 may be a therapeutic target for ovarian cancer.

• 出版日期2017-10
• 单位山东中医药大学