Dehydroepiandrosterone and metformin regulate proliferation of murine T lymphocytes

作者：Solano M E; Sander V; Wald M R; Motta A B^*

来源：Clinical and Experimental Immunology, 2008, 153(2): 289-296.

DOI：10.1111/j.1365-2249.2008.03696.x

• dehydroepiandrosterone
• metformin
• polycystic ovary syndrome
• proliferation
• T lymphocytes

The aim of the present study was to assess the effect of dehydroepiandrosterone (DHEA: 10 mu M) and metformin (10 mu M and 100 mu M) in regulating proliferation of cultured T lymphocytes. T cells were isolated from lymph nodes of prepuberal BALB/c mice. We found that DHEA, metformin and DHEA + metformin added to the incubation media diminished proliferation of T cells. The inhibition by DHEA was higher than that produced by metformin, while the combined treatment showed a synergistic action that allowed us to speculate distinct regulatory pathways. This was supported later by other findings in which the addition of DHEA to the incubation media did not modify T lymphocyte viability, while treatment with metformin and DHEA + metformin diminished cellular viability and increased both early and late apoptosis. Moreover, DHEA diminished the content of the anti-oxidant molecule glutathione (GSH), whereas M and DHEA + metformin increased GSH levels and diminished lipid peroxidation. We conclude that DHEA and metformin diminish proliferation of T cells through different pathways and that not only the increase, but also the decrease of oxidative stress inhibited proliferation of T cells, i.e. a minimal status of oxidative stress, is necessary to trigger cellular response.

• 出版日期2008-8