miR-24 Reduces Serum Lipid Levels and Inhibits Brain Tissue Cells Apoptosis of Rats with Cerebral Infarction

作者：Jiang, Qiyong; Shen, Dawei; Wang, Yihua; Huang, Dezhang; Wang, Zhigang

来源：Turkish Neurosurgery, 2020, 30(4): 483-490.

DOI：10.5137/1019-5149.JTN.23127-18.2

• Cerebral infarction
• miR-24
• Blood lipid
• Area of cerebral infarction
• Apoptosis
• Rat

AIM: To study miR-24 effects on cerebral infarction in rats. @@@ MATERIAL and METHODS: A rat middle cerebral artery occlusion model (MCAO) was constructed. Intracerebroventricular stereotactic injection of miR-24 agomir/antagomir was performed in the rat MCAO model. According to different experiences, rats were divided into normal, sham, MCAO, miR-24 agomir and miR-24 antagomir groups. Serum TCH, HDL and TG levels were detected. @@@ RESULTS: Comparingthe normal and sham groups, we observed decreased relative miR-24 expression (p<0.05) and increased cerebral infarction area percentage, apoptotic cells and relative caspase-3 protein expression (p<0.05) in theMCAO, miR-24 agomirand miR-24 antagomir groups. TC, TG and HDL-C levels of the MCAO and miR-24 antagomir groups were higher than those of normal and sham groups (p<0.05).Compared with the MCAO group, increased relative miR-24 expression (p<0.05) and decreased TC, TG and HDL-C levelscerebral infarction area percentage, number of apoptotic cells and caspase-3 expression (p<0.05) were found in themiR-24 agomir group, contrasting with theobservations from the miR-24 antagomir group. @@@ CONCLUSION: miR-24 reduced serum TCH, HDL and TG levels and inhibited brain tissue cell apoptosis in rats with cerebral infarction.

• 出版日期2020
• 单位山东大学