A development of LC-MS method combining ultrafiltration and lyophilization for determination of r-RGD-Hirudin in human serum

作者：Su Shengmin; Yu Yunqiu^*; Mo Wei; Zhang Yanling; Song Houyan; Chen Qinfen; Xie Yi

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2008, 870(1): 27-31.

DOI：10.1016/j.jchromb.2008.05.032

• r-RGD-Hirudin
• quantitative assay
• LC-MS
• ultrafiltration
• freeze drying
• clinical research

A reliable and validated LC-MS method was established for determination of r-RGD-Hirudin in human serum. Ultrafiltration was used instead of liquid-liquid extraction or solid phase extraction for water solubility drug r-RGD-Hirudin extraction. Freeze drying was used for concentration. The experiment conditions, including pre-processing procedure and LC-MS, have been investigated and optimized. Comparing with reported assays, the current method showed significant improvement in specificity, linearity, precision and sensitivity. This method has been successfully applied in clinical research of r-RGD-Hirudin.

• 出版日期2008-7-1
• 单位复旦大学