摘要

The effects of seed soaking with gibberellic acid (GA(3)) on germinability of conventional corn variety Nongda 108' and super sweet corn variety Chaotian 3' were investigated under deep-sowing condition. GA(3) soaking significantly improved seed germination and mesocotyl elongation of both varieties under 10 or 6cm sowing depth. The most significant promotion occurred at 0.175gL(-1) GA(3) in Nongda 108' and 0.035gL(-1) GA(3) in Chaotian 3'. To further illustrate the physiological explanations, seed respiration was estimated from dehydrogenase activity test and oxygen-sensing test. Dehydrogenase activities of both varieties were dramatically promoted after GA(3) soaking. Compared with water soaking, the dehydrogenase activity in 0.175gL(-1) GA(3)-soaked seeds of Nongda 108' and 0.035gL(-1) GA(3)-soaked ones of Chaotian 3' was increased by 15.4% and 78.8%, respectively. Oxygen-sensing test showed that increased metabolism time (IMT) critical oxygen pressure (COP) and relative germination time (RGT) were decreased while oxygen metabolism rate (OMR) and relative germination rate (RGR) were increased in GA(3)-soaked seeds, suggesting that exogenous GA-accelerated seed respiration. Our results suggested that seed soaking with exogenous GA(3) was a simple and practicable method to improve deep-sowing tolerance during corn seed germination, and the significant promotions were attributed to vigorous respiratory metabolism.