Direct Electrochemistry of Hemoglobin Immobilized in the Sodium Alginate and SiO2 Nanoparticles Bionanocomposite Film on a Carbon Ionic Liquid Electrode

作者：Sun W^*; Wang D; Zhai Z; Gao R; Jiao K

来源：Journal of the Iranian Chemical Society, 2009, 6(2): 412-419.

DOI：10.1007/bf03245852

• 1-Butyl-3-methylimidazolium hexafluorophosphate
• Hemoglobin
• Direct electron transfer
• Carbon paste electrode
• SiO2 nanoparticle

In this paper a carbon ionic liquid electrode (CILE) was fabricated by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) as binder. By using the CILE as basal electrode, the hemoglobin (Hb) molecule was immobilized on the surface of CILE with a sodium alginate (SA) hydrogel and SiO2 nanoparticles organic-inorganic composite material. The direct electrochemical behaviors of Hb in the bionanocomposite film were further studied in a pH 7.0 Britton-Robinson (B-R) buffer solution. A pair of well-defined quasi-reversible cyclic voltammetric peaks of Hb was obtained on SA/nano-SiO2/Hb/CILE with the formal potential (E-0') at -0.355 V (vs. SCE), which was the characteristic of heme Fe(III)/Fe(II) redox couples. The formal potential of Hb Fe(III)/Fe(II) couple shifted negatively with increasing pH of solution with a slope of -45.2 mV/pH, which indicated that a one electron transfer accompanied with one proton transportation. The immobilized Hb showed good electrocatalytic manner to the reduction of trichloroacetic acid (TCA).

• 出版日期2009-6
• 单位青岛科技大学