Adipose-derived stromal cell transplantation for treatment of stress urinary incontinence

作者：Wu, GuiZhu; Song, YanFeng^*; Zheng, Xiu; Jiang, ZhongQing

来源：Tissue and Cell, 2011, 43(4): 246-253.

DOI：10.1016/j.tice.2011.04.003

• Adipose-derived stem cells
• Injection
• Stress urinary incontinence
• Stem cell transplantation
• Cell therapy
• Urethra

We aimed to investigate the application of adipose-derived stromal cells in the treatment of stress urinary incontinence (SUI). Animal models of stress urinary incontinence were established with Sprague-Dawley female rats by complete cutting of the pudendal nerve. Rat adipose-derived stromal cells were isolated, cultured and successfully transplanted into animal models. Effects of stem cell transplantation were evaluated through urodynamic testing and morphologic changes of the urethra and surrounding tissues before and after transplantation. Main urodynamic outcome measures were measured. Intra-bladder pressure and leak point pressure were measured during filling phase. Morphologic examinations were performed. Transplantation of adipose-derived stem cells significantly strengthened local urethral muscle layers and significantly improved the morphology and function of sphincters. Urodynamic testing showed significant improvements in maximum bladder capacity, abdominal leak point pressure, maximum urethral closure pressure, and functional urethral length. Morphologic changes and significant improvement in urination control were consistent over time. It was concluded that periurethral injection of adipose-derived stromal cells improves function of the striated urethral sphincter, resulting in therapeutic effects on SUI. Reconstruction of the pelvic floor through transplantation of adipose-derived cells is a minimally invasive and effective treatment for SUI.

• 出版日期2011-8
• 单位中国人民解放军南京军区福州总医院; 福建医科大学