Molecular Screening of Abaca (Musa textilis L. Nee) Accessions Using Microsatellite Markers Associated With Resistance to Bunchy Top Virus

作者：Boguero Aaron Paul B; Parducho Mark Anthony L; Mendoza Mariecris Rizalyn D R; Abustan Mary Ann M; Lalusin Antonio G^*

来源：Philippine Journal of Crop Science, 2016, 41(2): 13-19.

• bunchy-top
• molecular markers
• principal component analysis (PCA)
• resistance
• Shannon-weaver diversity index

Fifty seven abaca accessions of the Institute of Plant Breeding were assessed using 6 SSR markers associated with resistance to determine their genetic diversity, identify resistant and susceptible accessions for bunchy top virus, and establish a basis for construction of a genetic base pool in breeding for resistance to bunchy top virus. Eighteen accessions were identified disease-free, 24 accessions showed mild resistance, 9 accessions were severely affected by bunchy top virus, and 6 accessions have no rating. All the accessions obtained from Palawan were observed healthy. The 57 accessions are highly diverse with Shannon-Weaver index of 0.92 and polymorphism information content value was 0.61. Accessions were grouped into 10 distinct groups with 41% Jaccard's similarity coefficient. Abuab Labo (resistant) and Maguindanao Pacol (resistant) have, 56% and 45% similarity to Pacol, respectively, a source of resistance, to bunchy top. Principal component analysis displayed a widely diverse set of population where each resistant accession is distributed throughout the four panels. The diversity of the accessions revealed that there would be other possible sources of resistance genes aside from Pacol which could be used for further improvement of abaca resistance.

• 出版日期2016-8