A 14-3-3 gamma dimer-based scaffold bridges CtBP1-S/BARS to PI(4)KIII beta to regulate post-Golgi carrier formation

作者：Valente Carman; Turacchio Gabriele; Mariggio Stefania; Pagliuso Alessandro; Gaibisso Renato; Di Tullio Giuseppe; Santoro Michele; Formiggini Fabio; Spano Stefania; Piccini Daniele; Polishchuk Roman S; Colanzi Antonino; Luini Alberto^*; Corda Daniela

来源：Nature Cell Biology, 2012, 14(4): 343-+.

DOI：10.1038/ncb2445

Large pleiomorphic carriers leave the Golgi complex for the plasma membrane by en bloc extrusion of specialized tubular domains, which then undergo fission. Several components of the underlying molecular machinery have been identified, including those involved in the budding/initiation of tubular carrier precursors (for example, the phosphoinositide kinase PI(4)KIII beta, the GTPase ARF, and FAPP2), and in the fission of these precursors (for example, PKD, CtBP1-S/BARS). However, how these proteins interact to bring about carrier formation is poorly understood. Here, we describe a protein complex that mediates carrier formation and contains budding and fission molecules, as well as other molecules, such as the adaptor protein 14-3-3 gamma. Specifically, we show that 14-3-3 gamma dimers bridge CtBP1-S/BARS with PI(4)KIII beta, and that the resulting complex is stabilized by phosphorylation by PKD and PAK. Disrupting the association of these proteins inhibits the fission of elongating carrier precursors, indicating that this complex couples the carrier budding and fission processes.

• 出版日期2012-4