Alkaline protease from senesced leaves of invasive weed Lantana camara

作者：Gaur Smriti; Wadhwa Neeraj^*

来源：African Journal of Biotechnology, 2008, 7(24): 4602-4608.

• Lantana camara
• senescenced leaves
• alkaline protease
• thermostability

Protease from senesced leaves of the weed Lantana camara was purified in a 2-step procedure involving ammonium sulfate precipitation and Sephadex G-250 gel permeation chromatography. The Sephadex-G-250 fraction of senesced leaves of Lantana camara showed 28.31 fold with a yield of 6.19%. The enzyme was shown to have a low molecular weight of 43 kda by SDS-PAGE. It was strongly activated by metal ions such as Cu(2+), Zn(2+), Mg(2+), Co(2+) and Mn(2+). It remained active at 60 degrees C, pH 10.5 even after 1 h of incubation when casein was used as substrate. The compatibility of the enzyme was studied with commercial and local detergents, 60% activity of the enzyme was retained even after 1 h of incubation at pH 10.0. The easy availability of the senesced leaves of this common weed makes it a cheaper enzyme source and potential additive in detergents.

• 出版日期2008-12-17