Microreplication and Design of Biological Architectures Using Dynamic-Mask Multiphoton Lithography

作者：Nielson Rex; Koehr Bryan; Shear Jason B^*

来源：Small, 2009, 5(1): 120-125.

DOI：10.1002/smll.200801084

• digital micromirror devices
• E. coli
• proteins
• tissue replication

A strategy for rapidly printing three-dimensional (3D) microscopic replicas using multiphoton lithography directed by a dynamic electronic mask is reported. Morphological descriptions of 3D structures are encoded as stacks of 2D slices created from tomographic and computer-designed instruction sets. In this manner, digital images serve as input for a sequence of reflective photomasks on a digital micromirror device to direct replication of a structure. By scanning a laser focus across the face of the intrinsically aligned masks, tomographic and computed data can be translated into protein-based 3D reproductions with submicrometer feature sizes within 1 min. This straightforward and highly versatile approach may provide improved routes for the development of 3D cellular scaffolds, rapid prototyping of microanalytical devices, and production of custom tissue replacements.

• 出版日期2009-1