Mitochondrial DNA Variations in Sibling Species of the Bulinus truncatus/tropicus Complex in Lake Albert, Western Uganda

作者：Nalugwa Allen^*; Kristensen Thomas K; Nyakaana Silvester; Jorgensen Aslak

来源：Zoological Studies, 2010, 49(4): 515-522.

• Bulinus truncatus/tropicus species complex
• Freshwater snails
• COI gene
• Genetic variation
• Lake Albert

Allen Nalugwa, Thomas K. Kristensen, Silvester Nyakaana, and Aslak Jorgensen (2010) Mitochondrial DNA variations in sibling species of the Bulinus truncatus/tropicus complex in Lake Albert, western Uganda. Zoological Studies 49(4): 515-522. Thirty-nine snail samples morphologically conforming to the Bulinus truncatus/tropicus species complex were collected from 5 populations (Booma, Bugoigo, Piida, Toonya, and Walukuba) on Lake Albert, western Uganda. Analysis of patterns of sequence variations and evolutionary relationships within and between localities using a 612 bp fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene revealed 5 unique haplotypes defined by 21 polymorphic sites. The Bayesian and median-joining network phylogenetic reconstructions partitioned the haplotypes into 2 reciprocally monophyletic clades concordant with B. truncatus and B. tropicus separated by 18 mutational substitutions, corresponding to a 3.0% net sequence divergence. Sequence divergences between haplotypes ranged 0.2%-3.5%, and significant genetic differentiation was observed in 5 of the 6 population pairwise comparisons. The 2 sibling species were sympatric in only 1 locality, at Piida. Our results confirm the existence of 2 evolutionary lineages of the Bulinus truncatus/tropicus species in Lake Albert and underscore the utility of the mitochondrial COI gene in differentiating between sibling species of the B. truncatus/tropicus complex which are otherwise indistinguishable based on the shell morphology. http://zoolstud.sinica.edu.tw/Journals/49.4/515.pdf

• 出版日期2010-7