Monitoring the efficacy of antimicrobial photodynamic therapy in a murine model of cutaneous leishmaniasis using L. major expressing GFP

作者：Latorre Esteves Elena; Akilov Oleg E; Rai Prakash; Beverley Stephen M; Hasan Tayyaba^*

来源：Journal of Biophotonics, 2010, 3(5-6): 328-335.

DOI：10.1002/jbio.201000008

• Leishmania
• photodynamic therapy
• murine model
• green fluorescent protein

A murine model of cutaneous leishmaniasis with green fluorescent protein positive (GFP+) L. major enables the monitoring of parasitic load via measurements of GFP fluorescence intensity, allowing for a faster and more efficient way of monitoring the clinical outcome of photodynamic therapy (PDT). This model may provide new insights on the phototoxic aspects in PDT. Although PDT regimens may be somewhat different in humans, it is expected that the developed model will facilitate the optimization and clinical translation of PDT as a therapy for cutaneous leishmaniasis and the eventual development of topical PDT treatments for other granulomatous infections.
[GRAPHICS]
Green fluorescence from GFP+ L. major in cutaneous leishmaniasis lesions on the ear of BALB/c mouse.

• 出版日期2010-6