Upregulation of Membrane-Bound CD40L on CD4(+) T cells in Women with Primary Sjogren's Syndrome

作者：Belkhir R; Gestermann N; Koutero M; Seror R; Tost J; Mariette X; Miceli Richard C^*

来源：Scandinavian Journal of Immunology, 2014, 79(1): 37-42.

DOI：10.1111/sji.12121

Epigenetic deregulation of genes encoded on the X chromosome as reported for CD40L in lupus could explain the female predominance of autoimmune diseases. We compared CD40L expression on CD4(+) T cells from primary Sjogren's syndrome (pSS) women and healthy controls and investigated DNA methylation patterns of the promoter and enhancer regions of CD40L. The expression of CD40L on activated CD4(+) T cells was higher in patients with pSS than controls after phorbolmyristate acetate and ionomycin activation (P=0.02). CD40L mRNA level in CD4(+) T cells did not differ between patients with pSS and controls and was similar in both groups in cultures treated with the demethylating agent 5-azacytidine C. Pyrosequencing analysis revealed no significant differences in methylation profiles between patients and controls. Inducible membrane-bound CD40L on CD4(+) T cells is increased in patients with pSS but was not related to epigenetic deregulation by demethylation patterns of the regulatory regions of CD40L.

• 出版日期2014-1
• 单位中国地震局