Does Dietary Deoxynivalenol Modulate the Acute Phase Reaction in Endotoxaemic Pigs?-Lessons from Clinical Signs, White Blood Cell Counts, and TNF-Alpha

作者：Tesch Tanja; Bannert Erik; Kluess Jeannette^*; Frahm Jana; Kersten Susanne; Breves Gerhard; Renner Lydia; Kahlert Stefan; Rothkoetter Hermann Josef; Daenicke Sven

来源：Toxins, 2016, 8(1): 3.

DOI：10.3390/toxins8010003

• pig
• deoxynivalenol
• lipopolysaccharide
• liver
• leukocytes
• clinical symptoms
• tumor necrosis factor alpha
• body core temperature

We studied the interaction between deoxynivalenol (DON)-feeding and a subsequent pre- and post-hepatic immune stimulus with the hypothesis that the liver differently mediates the acute phase reaction (APR) in pigs. Barrows (n = 44) were divided into a DON-(4.59 mg DON/kg feed) and a control-diet group, surgically equipped with permanent catheters pre-(V. portae hepatis) and post-hepatic (V. jugularis interna) and infused either with 0.9% NaCl or LPS (7.5 mu g/kg BW). Thus, combination of diet (CON vs. DON) and infusion (CON vs. LPS, jugular vs. portal) created six groups: CON_CONjug.-CONpor., CON_CONjug.-LPSpor., CON_LPSjug.-CONpor., DON_CONjug.-CONpor., DON_CONjug.-LPSpor., DON_LPSjug.-CONpor.. Blood samples were taken at -30, 15, 30, 45, 60, 75, 90, 120, 150, 180 min relative to infusion and analyzed for leukocytes and TNF-alpha. Concurrently, clinical signs were scored and body temperature measured during the same period. LPS as such induced a dramatic rise in TNF-alpha (p < 0.001), hyperthermia (p < 0.01), and severe leukopenia (p < 0.001). In CON-fed pigs, an earlier return to physiological base levels was observed for the clinical complex, starting at 120 min post infusionem (p < 0.05) and persisting until 180 min. DON_LPSjug.-CONpor. resulted in a lower temperature rise (p = 0.08) compared to CON_LPSjug.-CONpor.. In conclusion, APR resulting from a post-hepatic immune stimulus was altered by chronic DON-feeding.

• 出版日期2016-1